Biochem/physiol Actions

Primary TargetN-myristoylated G-protein βγ selective binding

Reversible: no

EC50 ~ 2.5 - 5 µM in activation of ERK1/2 in rat arterial smooth muscle and Rat2 cells

Product does not compete with ATP.

Cell permeable: yes

General description

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes α subunit dissociation without stimulating nucleotide exchange. Reported to cause a rapid activation of ERK1/2 (EC₅₀ ~2.5-5 µM) in RASM (rat arterial smooth muscle) and Rat2 cells. Promotes the phosphorylation of JNK and p38 MAP kinase. Also increases phospholipase C activity and causes Ca²⁺ release from internal stores.

A cell-permeable, N-myristoylated G-protein βγ selective binding peptide that disrupts the interaction between α and βγ subunits and promotes the α subunit dissociation without stimulating nucleotide exchange. Shown to cause a rapid activation of ERK1/2 (EC₅₀ ~ 2.5 - 5 µM) in rat arterial smooth muscle and Rat2 cells. Also promotes the phosphorylation of JNK and p38 MAP kinase, phospholipase C and causes Ca²⁺ release from internal stores. Negative control is also available (Cat. No. 371820).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1, 5 mg in Glass bottle

Packaged under inert gas

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C

Sequence

Myr-N-Ser-Ile-Arg-Lys-Ala-Leu-Asn-Ile-Leu-Gly-Tyr-Pro-Asp-Tyr-Asp-OH

Warning

Toxicity: Standard Handling (A)